This video demonstrates the essential steps for operating a 10x Genomics Chromium instrument for single-cell or molecular analysis. It outlines the process of preparing a microfluidic chip by loading reagents and attaching a gasket, inserting the prepared chip into the Chromium instrument, initiating the automated run, and finally, retrieving the generated "gems" for downstream applications like PCR.
Operating the 10x Genomics Chromium Instrument: A Step-by-Step Guide
0:00:07 Chip Preparation: The process begins with loading reagents into the microfluidic chip and attaching the gasket, preparing it for the instrument.
0:00:10 Instrument Compatibility: The demonstrated workflow is compatible with both the Chromium X and Chromium Controller instruments.
0:00:21 Chip Loading: The assembled chip and gasket are carefully placed horizontally into the Chromium instrument's tray after pressing the eject button.
0:00:37 Run Initiation: The tray is retracted, the instrument automatically identifies the chip configuration (e.g., "Chromium Chip G"), and the run is initiated by pressing the play button.
0:00:46 Run Duration: The automated run inside the Chromium instrument takes approximately 18 minutes to complete.
0:00:50 Post-Run Recovery: After the run, the chip is removed, and the "gems" (encapsulated cells or molecules) are transferred from the recovery wells into a PCR strip tube for subsequent processing.