This video introduces the Roche Avenio Edge system, a fully automated instrument designed for Next-Generation Sequencing (NGS) Library preparation, PCR Target enrichment, Library cooling, and quantification. Powered by Roche Kapa reagents, the system aims to simplify complex and laborious library prep protocols, making advanced genomic data accessible to more labs by reducing the need for highly trained automation experts and intricate software. Key features highlighted include an intuitive setup process, support for various sample input types, automated barcode scanning, prepackaged and barcoded reagents and consumables, intelligent inventory checks, pressure-sensitive pipetting and gripping for error minimization, a proprietary bead processing station for DNA maximization, an integrated quantification module, and temperature-controlled storage for processed samples. The system offers a true "walkaway" experience, significantly reducing manual intervention and allowing users to focus on experimental design and data analysis.
Exploring the Roche Avenio Edge System: Automated NGS Workflows
1:55 Introduction to Avenio Edge: The Avenio Edge system is presented as a fully automated solution for NGS Library preparation, PCR Target enrichment, Library cooling, and quantification, powered by Roche Kapa reagents.
2:20 Addressing Lab Challenges: The system is designed to overcome the difficulties of laborious and finicky library prep protocols, reducing the need for specialized automation experts and complex software, thereby lowering barriers to sequencing.
2:41 Easy Setup - Sample Loading: Users begin by placing samples (supporting MagnaPure 2/4/8 tube strips, high/low profile MagnaPure 96 plates, and Avenio Edge process plates) onto designated highlighted locations.
3:05 Automated Barcode Scanning & Protocol Selection: An on-deck barcode reader scans sample inputs, retrieving order information from a host system or allowing manual protocol and sample data entry via the touchscreen.
3:27 Guided Reagent & Consumable Loading: The software guides the user on loading necessary reagents and consumables. The system accepts prepackaged reagents in mini racks, foil-sealed troughs, cooled tube racks, and barcoded cartridges to minimize manual touchpoints and errors.
4:08 Intelligent Tip Management: Avenio Edge tips are liquid-level sensing, filtered, DNA/RNA-free, and barcoded, allowing for random placement, tracking, and even reuse of partially used tip racks (via a specific consumable for appropriate situations).
4:37 Automated Inventory Check & Error Correction: After loading, a fully automated inventory check verifies correct resource placement. If an error is detected, the touchscreen precisely indicates the location for correction, followed by a re-scan.
4:50 Temperature Control for Reagents: Temperature-sensitive reagents (enzymes, master mixes) are automatically transferred to an on-deck cooling station to maintain optimal conditions.
5:54 Advanced Verification Features: Beyond barcode scanning, the system uses pressure-sensitive pipettes to ensure all tube caps are removed and pressure-sensitive grippers to confirm proper labware placement, minimizing human error.
6:30 Core Automation Hardware: The system features an 8-channel pipetting arm, an integrated quantification module (samples quantified below deck), and a gripper finger exchange station for handling items below deck.
7:11 Proprietary Bead Processing: A unique bead processing station uses a magnet that moves along plate wells to precisely capture beads, maximizing DNA recovery.
8:03 Post-Run Sample Management: Upon completion, samples are moved to a temperature-controlled storage location (up to 72 hours) and covered until retrieved by the user.
8:44 Comprehensive Run Report: After the run, the touchscreen displays a detailed report, including pass/fail information and sample concentrations, which can be exported or sent to a host system.
8:58 "Walkaway" Experience & Simplicity: The system is highlighted for its "true walkaway" experience, enabling users to set up a 24-sample run (library construction through target enrichment) in under 20 minutes without further intervention. It features only one power button, an intuitive touchscreen, and integrated storage.
9:47 Impact on Lab Productivity: By automating laborious steps, Avenio Edge allows researchers to dedicate more time to critical tasks like experimental design, data analysis, and patient-focused decisions.