This video highlights the advantages of Roche's Avenio Edge liquid handling system compared to traditional liquid handlers for molecular pathology labs, particularly in running cancer research panels and Next-Generation Sequencing (NGS) workflows. It showcases the Avenio Edge's user-friendly features, including a touchscreen interface with pre-set methods, self-guided instructions for labware loading, pre-filled barcoded reagent cartridges, and an integrated 'load check' system for error prevention. A key differentiator is its on-deck quantification module, which facilitates seamless normalization and allows multiple panels to be run simultaneously within a single protocol. This contrasts significantly with traditional systems that demand manual master mix preparation, off-deck quantification, laborious normalization steps, and sequential single-panel runs, leading to increased technician workload and potential for errors. The Avenio Edge further streamlines operations with 21 CFR Part 11 compliant reporting, enhancing workflow automation and reliability for in-house NGS.
Comparing Avenio Edge vs. Traditional Liquid Handlers for Molecular Pathology Labs
0:05 System Comparison Introduction: The video introduces a comparison between the Avenio Edge system and a traditional liquid handler, demonstrating their use by two technicians, Ed and Bill, for running cancer research panels.
0:33 Effortless Method Loading: Ed, using the Avenio Edge, easily loads a multi-panel method from a touchscreen's drop-down menu, while Bill on the traditional system spends time searching through multiple PC folders.
1:06 Self-Guided & Barcoded Setup: The Avenio Edge provides self-guided visual instructions for loading pre-filled reagent cartridges, sample plates, and other barcoded labware, eliminating the need for manual Master Mix preparation and ensuring reliable sample tracking.
2:10 Automated Load Check: The Avenio Edge features an automated "load check" process that scans barcoded labware for correct placement and orientation, alerting Ed immediately to any errors, misplacements, or forgotten items.
2:48 Traditional System Limitations: Traditional liquid handlers often rely on less robust camera-based error checking, which may fail to detect if the correct labware is present or properly oriented, potentially leading to downstream issues.
3:05 Rapid Setup and Hands-Off Operation: Ed completes his run setup on the Avenio Edge in approximately 20 minutes, allowing him to start the run and move on to other tasks or leave for the day.
3:14 On-Deck Quantification & Multi-Panel Runs: The Avenio Edge includes an integrated on-deck quantification module for seamless normalization and pooling calculations. It can run multiple panels simultaneously within the same protocol, allowing users to select specific panels for different samples.
3:45 Increased Manual Workload for Traditional Systems: Bill, using a traditional system, must manually label labware, prepare master mixes, stay to perform off-deck quantification, conduct tedious normalization calculations, and reload the instrument for each single-panel run.
4:36 Compliant Reporting: The Avenio Edge generates 21 CFR Part 11 compliant run reports accessible on its touchscreen, simplifying audit trails by automatically tracking lot numbers and run parameters, which can be exported as a PDF.
4:46 End-to-End NGS Solution: The Avenio Edge is presented as an end-to-end IVD liquid handler for NGS Library Prep and Target Enrichment, ideal for molecular pathology labs looking to bring NGS workflows in-house.
5:23 Key Differentiators: Its integrated on-deck quantification and ability to handle multiple cancer research panels in a single, end-to-end NGS workflow are highlighted as significant advantages, addressing bottlenecks faced by other traditional liquid handlers.